Di-(N-succinimidyl)-3,3´-dithiodipropionate 1 g

Dithiobis(succinimidyl propionate) (DSP or DTSP) is a homobifunctional crosslinker that is cell membrane permeable. DSP has amine-reactive N-hydroxysuccinimide (NHS) esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm. DSP contains a reducible disulfide (thiol) bond in the spacer arm that make it a good choice for various applications.

DSP must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous crosslinking reaction. DSP can be used to crosslink intracellular proteins. If extracellular crosslinking is desired, then DTSSP, the water soluble analog of DSP can be used.