Urea crosslinker – C4-arm, NHS ester (DSBU, BuUrBU) 50 mg

Ureido-4,4´-dibutyric acid bis(hydroxysuccinimide) ester, also known as DSBU or BuUrBU, is the longest length version of MS-cleavable urea-based lysine-lysine reactive homobifunctional cross-linkers. At neutral or slightly basic pH, it irreversibly crosslinks the neighbouring lysine groups. The presence of the symmetrical urea moiety which is prone to collision-induced dissociation allows to perform unambiguous distinguishing of crosslinks in tandem CID-MS experiments.

The urea-based MS-cleavable crosslinkers are based on the concept pioneered by Prof. Dr. Andrea Sinz of the Halle University.