anti-Synaptophysin mouse monoclonal, SY38, 0.2 ml
SKU 1NN3.1
€ 110,31
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anti-Synaptophysin mouse monoclonal, SY38, 0.2 ml
Product Details
CAS number: 72-18-4
Chemical formulas: C5H11NO2
HS code: 30021500
Brand: PROGEN
anti-Synaptophysin mouse, monoclonal, SY38, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Synaptophysin mouse monoclonal, SY38 50 μg/ml, monoclonal, affinity purified
SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas.
Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid.
Polypeptide reacting: 38 kDa transmembrane glycoprotein of presynaptic vesicles.
Tested cultured cell lines: rat PC-12 cell line.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: SY38
Immunogen: synaptophysin from presynaptic vesicles, prepared from bovine brain
UniprotID: P20488 (bovine), P08247 (human), Q62277 (mouse), P07825 (rat)
Synonym: synaptophysin, major synaptic vesicle protein p38, SYP
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, human, mouse, rat
Toepassing
Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:50-1:200 (0.25-1 μg/ml, no protease treatment)
Immunohistochemistry (IHC) - paraffin: 1:50-1:200 (0.25-1 μg/ml, microwave treatment recommended, no protease treatment)
Western Blot (WB): 1:500-1:1,000 (0.05-0.1 μg/ml)
Mouse brain (courtesy of J. Heß, University Hospital Heidelberg)
Western blot analysis of mouse brain lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg mouse brain lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Western blot analysis of rat Ins-1 lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg rat Ins-1 lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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