SYBR® Green DNA dye, 1.8 ml, 1 x 1.8 ml
SKU 1CN2.1
€ 30,98
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SYBR® Green DNA dye, 1.8 ml, 1 x 1.8 ml
Product Details
HS code: 38229000
Brand: Carl Roth
SYBR Green DNA dye,, for electrophoresis, ready-to-use, 11x concentrated, Density (D) 1 g/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C
This is the DNA dye SYBR® Green I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/loading buffer mixture and then loaded onto the gel.
SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
- Recommended for dsDNA
- Direct addition to DNA sample
- For excitation with UV light and blue light
- Non-toxic alternative for ethidium bromide
- Sensitivity (0,01 ng/band)
SYBR® Green DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.
Golflengte
Excitation maximum (bound to DNA): approx. 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm
SYBR® Green DNA dye 11x conc., for electrophoresis, ready-to-use
Technische informatie
| Activeringsmax. (DNA-gebonden) | 254 nm 495 nm |
| Aantal sporen | ca. 2000/ml |
| Emissiemax. (DNA-gebonden) | 521 nm |
| Sensitiviteit | 0,01 ng/band |
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
| Appearance | orange solution |
| Each batch is functionally tested for its suitability in electrophoresis. | |
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